Figure 4From: A DIVA vaccine strain lacking RpoS and the secondary messenger c-di-GMP for protection against salmonellosis in pigsVaccinated piglets exhibited significant levels of serum IgG against Salmonella at the time of challenge. Sera from the control and vaccinated group were pooled into four samples per day. A Sera were analyzed by Western blot against a hot saline antigenic extract of Salmonella ΔXIII strain. In this case, an equal volume of each pool and group was mixed and the resulting samples were used for analysis. C: control, V: vaccinated. B Pooled sera were analyzed by an LPS based IDEXX test. An S/P ratio of ≥ 0.25 is considered positive. Statistical analysis was carried out using a two-way analysis of variance combined with the Bonferroni test. **P < 0.01.Back to article page