Figure 10

PLG binding ability of rTsENO and M-rTsENO, as determined by ELISA. ELISA plates were coated with rTsENO (0.6 μg/well), M-rTsENO (0.6 μg/well), ScENO (0.4 μg/well, positive control) or BSA (0.8 μg/well, negative control). Then, the wells were incubated with various concentrations of human PLG (0.05-2.0 µg/mL). An anti-PLG antibody was utilized to detect PLG under standard ELISA protocols. The ability of PLG to bind with rTsENO, M-rTsENO and ScENO was dose-dependent. The dots on the different lines indicate the mean absorbance values at 450 nm \(({\bar{\text{x}}} \pm {\text{SD}}).\)