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Table 1 Bacterial strains and plasmids used in this study

From: Lsr operon is associated with AI-2 transfer and pathogenicity in avian pathogenic Escherichia coli

Strains or plasmidRelevant genotype and propertySource or references
Escherichia coli strains
 BL21DE3Invitrogen
 BL21ΔluxSBL21 DE3luxS deletion mutant strain[23]
 MG1655 [16]
 DE17APEC serotype O2[2]
 DE17ΔluxSΔpfsDE17 luxS and pfs mutant strainThis study
 APEC94Wide type, isolated from sick chicken (serotype O78)This study
 APEC94Δlsr(Cm)Lacking of lsr operonThis study
Salmonella strains
 Salmonella typhimuriumLT2ATCC 14028
Vibrio harveyi strains
 BB170BB120 luxN::Tn5 (sensor 1−, sensor 2 +)[13]
 BB152BB120 luxM::Tn5 (representing AI-2)[21]
Plasmids
 pKD46The vector containing arabinose-inducible phage λ Red recombinase (AmpR)[17]
 pACYC177-lsrThe lsr operon complement plasmidThis study
 pCold TFExpression vector with AmpRTaKaRa
 pKD3The plasmid contained chloramphenicol resistance (CmR)[17]
 pCold TF-lsrBRecombinant plasmid contained lsrB of APECThis study