Table 1 Bacterial strains and plasmids used in this study
From: Lsr operon is associated with AI-2 transfer and pathogenicity in avian pathogenic Escherichia coli
Strains or plasmid | Relevant genotype and property | Source or references |
|---|---|---|
Escherichia coli strains | ||
 BL21 | DE3 | Invitrogen |
 BL21ΔluxS | BL21 DE3luxS deletion mutant strain | [23] |
 MG1655 |  | [16] |
 DE17 | APEC serotype O2 | [2] |
 DE17ΔluxSΔpfs | DE17 luxS and pfs mutant strain | This study |
 APEC94 | Wide type, isolated from sick chicken (serotype O78) | This study |
 APEC94Δlsr(Cm) | Lacking of lsr operon | This study |
Salmonella strains | ||
 Salmonella typhimurium | LT2 | ATCC 14028 |
Vibrio harveyi strains | ||
 BB170 | BB120 luxN::Tn5 (sensor 1−, sensor 2 +) | [13] |
 BB152 | BB120 luxM::Tn5 (representing AI-2) | [21] |
Plasmids | ||
 pKD46 | The vector containing arabinose-inducible phage λ Red recombinase (AmpR) | [17] |
 pACYC177-lsr | The lsr operon complement plasmid | This study |
 pCold TF | Expression vector with AmpR | TaKaRa |
 pKD3 | The plasmid contained chloramphenicol resistance (CmR) | [17] |
 pCold TF-lsrB | Recombinant plasmid contained lsrB of APEC | This study |