Visualizing bovine leukemia virus (BLV)-infected cells and measuring BLV proviral loads in the milk of BLV seropositive dams

Table 2 Detection of the proviral load in blood and milk samples

Sample	Age (years)	WBC^a (10²/µL)	LYMPH^b (10²/µL)	SCC^c (10⁶/mL)	BLV antibodies^e	Proviral load^f (copies/10⁵ cells)
Sample	Age (years)	Blood		Milk	Plasma	Blood	Milk
BLV-free cows
K1	2	NT^d	NT	0.8	–	0	0
K2	2	NT	NT	1.3	–	0	0
BLV-infected cows without lymphoma
Y2	5	103	53	NT	+	0	0
N6	2	99	38	NT	+	0	0
N7	3	79	23	NT	+	0	0
Y3	7	96	38	NT	+	51	0
G14	6	59	12	NT	+	54	0
S7	9	61	28	NT	+	61	0
S6	4	91	38	NT	+	68	0
K3	3	120	41	1.7	+	112	0
S5	2	80	42	NT	+	361	0
K4	5	69	41	1.6	+	390	0
K5	5	74	34	1.6	+	578	32
G9	2	108	75	NT	+	1457	0
S4	7	77	37	NT	+	3622	0
G11	4	90	16	NT	+	6389	0
N5	7	104	52	2.3	+	10 169	76
G12	6	137	18	NT	+	10 480	0
G10	2	152	26	NT	+	12 097	0
S3	5	105	53	NT	+	17 130	26
G5	4	118	53	NT	+	18 000	32
G8	6	113	54	NT	+	18 793	7
K6	3	78	19	NT	+	19 548	19
S1	7	109	60	NT	+	20 139	0
K7	3	119	68	6.4	+	21 267	0
G4	8	114	43	NT	+	21 284	51
G2	6	85	47	NT	+	21 603	0
G13	3	116	NT	NT	+	22 331	0
G7	3	87	16	NT	+	24 387	0
GDF211	8	82	45	NT	+	24 694	56
G6	6	104	61	NT	+	25 139	70
S2	3	150	94	NT	+	26 002	0
N4	6	93	33	NT	+	27 642	0
Y1	8	72	28	NT	+	34 831	64
GDF349	6	142	83	NT	+	35 859	66
N2	4	158	92	NT	+	35 758	83
N1	3	86	38	NT	+	37 707	159
N3	2	134	89	2.0	+	39 024	0
G3	7	106	45	NT	+	39 237	43
K8	3	175	120	1.2	+	53 985	179
K9	4	214	131	NT	+	58 734	0
G1	5	168	105	9.1	+	65 000	95
K10	5	206	140	1.2	+	72 014	54
K11	7	NT	NT	0.5	+	78 629	129
K12	4	NT	NT	1.0	+	84 479	47
BLV-infected cows with lymphoma
A1	8	109	NT	4.5	+	4500	6340
A2	6	196	157	2.5	+	65 782	422
A3	5	NT	NT	NT	+	111 564	125

^aWBC: White blood cell.
^bLYMPH: lymphocyte.
^cSCC: somatic cell count.
^dNT: not tested.
^eELISA testing was performed using an anti-BLV ELISA kit (JNC Inc.) targeting BLV gp51. +, positive for anti-BLV antibodies; −, negative for anti-BLV antibodies.
^fProviral loads per 10⁵ cells in blood and milk were measured via BLV-CoCoMo-qPCR-2 assay (RIKEN Genesis) [40], using THUNDERBIRD Probe qPCR Mix (Toyobo).

ISSN: 1297-9716