Figure 4
PEDV + VA litters had increased survival rates and decreased PEDV RNA shedding titers. A Kaplan–Meier survival curve of mock, mock + VA, PEDV and PEDV + VA litters at post-challenge day (PCD) 0–14. B Normalized weight gain of litters at PCD 0–15. Weights were normalized by dividing the daily weight (lbs) by PCD 0 weight (lbs). C Diarrhea was determined by fecal consistency score > 1 (fecal consistency was scored as follows: 0, normal; 1, pasty/semiliquid; 2, liquid; 3, watery). D Virus shedding was determined by real time quantitative polymerase chain reaction (RT-qPCR) and expressed as log10 copies/mL. Piglet diarrhea scores and viral shedding were measured at PCD 1–7, 9, 11, 13, and 15. Different alphabetical letters indicate significant differences among treatment groups at the same time point (mean ± SEM). Statistical analysis was performed using the log-rank (Mantel-Cox) test (A) or the two-way ANOVA with repeated measures and Bonferroni’s correction for multiple comparisons (B–D).