Bacterial dissemination in fish tissues. Flounders were infected with the same dose of TX01, TX01ΔhutZ, or TX01ΔhutZC, and bacterial recovery from the spleen and kidney was determined by plate counting at 24 h and 48 hpi. Data are presented as the means ± SEMs (N = 3). N, the number of times the experiment was performed. *P < 0.05, **P < 0.01.