Figure 4

Effects of norV on Hcp protein expression. A The Hcp proteins in the whole cells of bacteria grown under aerobic and anaerobic conditions exposing to various concentrations of SNP were determined by Western blot. Polyclonal anti-Hcp antibodies were used to measure the production of Hcp and anti-GroEL antibodies served as internal reference. Lines 1, 4, 7, 10 represent the wild type strain NJ-35; Lines 2, 5, 8, 11 represent the ΔnorV mutant; and Lines 3, 6, 9, 12 represent the complemented CΔnorV strain. The relative changes of Hcp production of bacteria cultured under aerobic (B) and anaerobic (C) conditions are given between the blots. The intensities of the Hcp and GroEL bands were measured and Hcp was equalized to that of GroEL. The relative mRNA levels of hcp gene in A. hydrophila strains grown under aerobic (D) and anaerobic (E) conditions after treated with various concentrations of SNP were determined by qRT-PCR. Values are the means ± SD from three biological replicates. *P < 0.05, **P < 0.01.