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Table 1 MAP culture results detected by F57-specific qPCR for fecal and tissue samples and INF-γ detection from whole blood samples

From: Quantifying transmission of Mycobacterium avium subsp. paratuberculosis among group-housed dairy calves

Pen

Calf ID

IN:CE

MAP detection with F57 qPCR following culture of individual fecal samples

Days following group housing

0

3

5

7

10

12

14

17

19

21

24

26

28

31

33

1

1

IN

I

 

C

I

 

C

 

C

  

C

I

I

 

C

1

2

IN

   

C

I

I

 

C

I

 

C

I

I

I

I

1

15

CE

       

C

I

      

1

16

CE

        

C

      

2

3

IN

 

C

I

I

  

C

I

I

I

I

I

I

 

C

2

4

IN

I

 

C

  

C

I

I

I

I

I

I

I

 

C

2

17

CE

   

C

 

C

I

      

C

I

2

18

CE

     

C

     

C

  

C

3

5

IN

    

C

 

C

I

 

C

I

I

I

I

I

3

6

IN

      

C

I

I

I

I

I

I

I

I

3

19

CE

     

C

I

        

3

20

CE

      

C

     

C

 

C

4

7

IN

I

  

C

I

   

C

I

I

 

C

  

4

8

IN

I

I

 

C

I

I

I

I

I

I

I

I

I

I

I

4

21

CE

           

C

 

C

I

4

22

CE

  

C

   

C

    

C

 

C

 

5

9

IN

   

C

I

  

C

I

I

  

C

I

I

5

10

IN

   

C

 

C

I

I

I

I

I

I

I

I

I

5

23

CE

             

C

 

5

24

CE

   

C

      

C

  

C

 

6

11

IN

     

C

 

C

 

C

   

C

 

6

12

IN

I

     

C

C

 

C

I

   

C

6

25

CE

        

C

    

C

 

6

26

CE

            

C

  

7

13

IN

 

C

   

C

  

C

I

I

 

C

 

C

7

14

IN

  

C

I

  

C

  

C

I

I

I

 

C

7

27

CE

    

C

  

C

  

C

  

C

 

7

28

CE

        

C

 

C

    

Pen

Calf ID

IN:CE

MAP detection with F57 qPCR following culture of individual fecal samples

Days following group housing

35

38

40

42

45

47

49

52

54

56

59

61

63

66

68

1

1

IN

I

I

I

I

I

I

I

I

I

      

1

2

IN

 

C

I

I

I

 

C

I

I

I

 

C

   

1

15

CE

C

I

  

C

 

C

        

1

16

CE

 

C

I

I

I

     

C

    

2

3

IN

I

I

I

I

I

I

I

      

C

 

2

4

IN

I

I

I

I

 

C

I

 

C

I

I

I

I

  

2

17

CE

I

I

I

          

C

 

2

18

CE

I

            

C

 

3

5

IN

I

I

I

I

I

I

I

I

I

I

I

I

I

I

I

3

6

IN

I

I

I

I

I

I

I

        

3

19

CE

  

C

            

3

20

CE

  

C

         

C

  

4

7

IN

C

I

I

     

C

 

C

    

4

8

IN

I

I

I

      

C

     

4

21

CE

  

C

     

C

      

4

22

CE

               

5

9

IN

  

C

I

I

I

I

I

I

I

 

C

   

5

10

IN

I

I

I

I

 

C

I

I

I

I

 

C

I

I

I

5

23

CE

   

C

           

5

24

CE

          

C

    

6

11

IN

C

     

C

   

C

    

6

12

IN

I

  

C

 

C

I

I

I

   

C

  

6

25

CE

       

C

       

6

26

CE

               

7

13

IN

I

I

I

I

 

C

I

I

I

      

7

14

IN

I

I

   

C

  

C

   

C

  

7

27

CE

 

C

        

C

    

7

28

CE

            

C

  

Pen

Calf ID

IN:CE

MAP detection with F57 qPCR following culture of individual fecal samples

INF-ƴ

Tissue culture

Days following group housing

70

73

75

77

80

82

84

87

89

91

94

96

101

1

1

IN

             

+

+

1

2

IN

             

+

+

1

15

CE

             

−

+

1

16

CE

      

C

      

−

−

2

3

IN

             

+

+

2

4

IN

             

+

+

2

17

CE

   

C

         

+

+

2

18

CE

       

C

     

+

+

3

5

IN

I

I

 

C

         

+

+

3

6

IN

 

C

           

+

+

3

19

CE

             

−

+

3

20

CE

      

C

      

+

+

4

7

IN

             

+

+

4

8

IN

             

+

+

4

21

CE

             

−

+

4

22

CE

 

C

           

+

−

5

9

IN

             

+

+

5

10

IN

I

 

C

I

   

I

     

+

+

5

23

CE

             

+

−

5

24

CE

             

−

−

6

11

IN

             

+

+

6

12

IN

C

            

+

+

6

25

CE

   

C

         

−

−

6

26

CE

C

     

C

      

−

+

7

13

IN

   

C

         

+

+

7

14

IN

             

+

+

7

27

CE

             

−

−

7

28

CE

     

C

       

−

−

  1. “I” indicate an infectious animal and “C” indicates a new shedding event. CE calves were individually housed 87 days following group housing (italic).
  2. CE contact exposed, IN inoculated.
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Veterinary Research

ISSN: 1297-9716