Tn-sequencing of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis mutant libraries reveals non-essential genes of porcine mycoplasmas differing in pathogenicity

Table 2 Two round PCR program

Step	°C	Time	Go to
1^st round PCR
1	95	4 min
2	95	30 s
4	42	30 s and slope −2 °C per cycle
5	72	1.5 min	Step 2, 6 times
6	95	30 s
7	55.8	30 s
8	72	1.5 min	Step 7, 20 times
9	72	5 min
2^nd round PCR
1	95	4 min
2	95	30 s
3	59	30 s
4	72	1 min	Step 2, 25 times
5	72	5 min

ISSN: 1297-9716