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Table 2 Two round PCR program

From: Tn-sequencing of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis mutant libraries reveals non-essential genes of porcine mycoplasmas differing in pathogenicity

Step °C Time Go to
1st round PCR
 1 95 4 min  
 2 95 30 s  
 4 42 30 s and slope −2 °C per cycle  
 5 72 1.5 min Step 2, 6 times
 6 95 30 s  
 7 55.8 30 s  
 8 72 1.5 min Step 7, 20 times
 9 72 5 min  
2nd round PCR
 1 95 4 min  
 2 95 30 s  
 3 59 30 s  
 4 72 1 min Step 2, 25 times
 5 72 5 min