Figure 2From: Tn-sequencing of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis mutant libraries reveals non-essential genes of porcine mycoplasmas differing in pathogenicityTn-sequencing schematic representation. A two round PCR followed by Illumina sequencing was done. The first round is done with a Tn-specific and arbitrary outside primers, the second round with nested primers. This PCR product is then Illumina-sequenced resulting in 36 bp target gene sequence.Back to article page