Skip to main content
Figure 5 | Veterinary Research

Figure 5

From: Matrine inhibits IL-1β secretion in primary porcine alveolar macrophages through the MyD88/NF-κB pathway and NLRP3 inflammasome

Figure 5

Matrine inhibited NLRP3 inflammasome activation in PAMs. A The mRNA expression of NLRP3, ASC and caspase-1 was detected by qRT-PCR. B, C Protein levels in PAMs were assessed with antibodies against NLRP3 and caspase-1 by immunoblotting (left panel). Quantification of the protein expression was performed by densitometric analysis of the bands (right panel). D Effects of matrine on ASC protein expression. Expression was normalized to GAPDH expression. E ASC specks (FITC) and nuclei (DAPI) were visualized using a confocal fluorescence microscope. ASC is evenly distributed in the cytoplasm and nucleus (control), and 5′UTR RNA and LPS co-stimulation induced the formation of ASC specks near the nuclear membrane. Matrine inhibited the formation of ASC specks. #Indicates that the expression was significantly different compared to that of the group with 5′UTR RNA and LPS co-stimulation (p < 0.05).

Back to article page

Veterinary Research

ISSN: 1297-9716