Figure 2
From: Mycoplasma hyopneumoniae evades phagocytic uptake by porcine alveolar macrophages in vitro
Overnight phagocytosis assay of M. hyopneumoniae. Porcine alveolar macrophages were incubated with M. hyopneumoniae or E. coli overnight (16 h) and then 400 µg/mL gentamicin was applied for 6 h. Samples were plated on to solid medium to enumerate bacteria. A M. hyopneumoniae recovered from culture supernatant or disrupted macrophages, with and without gentamicin treatment. B E. coli recovered from culture supernatant or disrupted macrophages, with and without gentamicin treatment. Error bars show standard error of the mean from one experiment performed in triplicate.