Toll-like receptor 5-mediated IL-17C expression in intestinal epithelial cells enhances epithelial host defense against F4+ ETEC infection

Luo, Yu; Xu, Jia; Zhang, Chaoying; Jiang, Chunyan; Ma, Yanfeng; He, Haijian; Wu, Yuan; Devriendt, Bert; Cox, Eric; Zhang, Hongbin

doi:10.1186/s13567-019-0665-8

Veterinary Research

Table 1 List of the primers used in the qPCR assay

From: Toll-like receptor 5-mediated IL-17C expression in intestinal epithelial cells enhances epithelial host defense against F4⁺ ETEC infection

Gene	Sequence (5′ → 3′)	Size (bp)	Ta (°C)	References
IL-17A	F: ACTCCAAACGCTTCACCTCAC	234	58	[17]
IL-17A	R: AGCCCACTGTCACCATCACTT	234	58	[17]
IL-17C	F: CGTGTGGACACGGATGAGAG	217	60	Present study
IL-17C	R: GGATGAACTCGGCGTGGAAG	217	60	Present study
IL-17RE	F: CCCAGATTCCTCGCCATACC	106	60	Present study
IL-17RE	R: CCTGGCAACAGATACAGGCA	106	60	Present study
TLR1	F: AGATTTCGTGCCACCCTATG	277	55	[50]
TLR1	R: CCTGGGGGATAAACAATGTG	277	55
TLR2	F: TGCTATGACGCTTTCGTGTC	163	55
TLR2	R: CGATGGAGTCGATGATGTTG	163	55
TLR3	F: GAGCAGGAGTTTGCCTTGTC	149	55
TLR3	R: GGAGGTCATCGGGTATTTGA	149	55
TLR4	F: TCATCCAGGAAGGTTTCCAC	234	55
TLR4	R: TGTCCTCCCACTCCAGGTAG	234	55
TLR5	F: GGTCCCTGCCTCAGTATCAA	114	55
TLR5	R: TGTTGAGAAACCAGCTGACG	114	55
TLR6	F: TCAAGCATTTGGACCTCTCA	170	58
TLR6	R: TTCCAAATCCAGAAGGATGC	170	58
TLR7	F: TCTGCCCTGTGATGTCAGTC	317	55
TLR7	R: GCTGGTTTCCATCCAGGTAA	317	55
TLR8	F: CTGGGATGCTTGGTTCATCT	241	55
TLR8	R: CATGAGGTTGTCGATGATGG	241	55
TLR9	F: AGGGAGACCTCTATCTCCGC	205	55
TLR9	R: AAGTCCAGGGTTTCCAGCTT	205	55
TLR10	F: GCCCAAGGATAGGCGTAAAT	128	55
TLR10	R: CTCGAGACCCTTCATTCAGC	128	55
pBD-2	F: CTGTCTGCCTCCTCTCTTCC	168	60	[51]
pBD-2	R: CAGGTCCCTTCAATCCTGTT	168	60	[51]
Claudin-1	F: ACCCGCACTACGTCACCTTC	146	60	Present study
Claudin-1	R: GGCAGGACACCTGGTCATTG	146	60	Present study
Claudin-2	F: AGAAGTTTCAAAGCCTGGGAG	119	60	[52]
Claudin-2	R: TCAACCTCATACAGGGGCAAA	119	60	[52]
Occludin	F: GTGGTAACTTGGAGGCGTCTTC	102	58	[53]
Occludin	R: CCGTCGTGTAGTCTGTCTCGTA	102	58
ZO-1	F: AAGGATGTTTACCGTCGCATT	253	60
ZO-1	R: ATTGGACACTGGCTAACTGCT	253	60
TNF-α	F:GCATGGTGGTGGTTGTTTCTGACGAT	99	60
TNF-α	R:GCTTCTGTTGGACACCTGGAGACA	99	60
IL-8	F: TCTCGGTGTAGAGCAAGG	146	58
IL-8	R: TTCCCAAAGTGCTGGTATT	146	58
β-Actin	F: TCATCACCATCGGCAACG	133	60	[54]
β-Actin	R: TTCCTGATGTCCACGTCGC	133	60	[54]
GAPDH	F: GGGCATGAACCATGAGAAGT	230	60	[55]
GAPDH	R: AAGCAGGGATGATGTTCTGG	230	60	[55]
RPL19	F: AACTCCCGTCAGCAGATCC	147	60	[56]
RPL19	R: AGTACCCTTCCGCTTACCG	147	60	[56]

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ISSN: 1297-9716

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