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Table 3 Primer sequences for real-time PCR

From: HcTTR: a novel antagonist against goat interleukin 4 derived from the excretory and secretory products of Haemonchus contortus

Target

NCBI Gene ID

Sequence of nucleotide (5′-3′)

Efficiencya

Tm (°C)

Amplicon size (bp)

Correlation coefficients (r2)

IL4R

102176382

CACGTCACCCACACATCATTA

AAACTCCGTCTCTTCCCATTC

1.01436

58.31

129

0.9897

JAK2

102180670

AGTGCCCGTGACTCATGAAA

AGATGTCCAGTGGCGTTTGA

1.107772

59.60

101

0.9977

STAT6

102180571

GGTTCAGTGACTCAGAGATTGG

GGCAGAGAATGGCTGGATATT

1.069265

58.41

98

0.9997

β-Actin

102179831

CACCACACCTTCTACAAC

TCTGGGTCATCTTCTCAC

1.070216

52.74

106

0.9958

  1. aAmplification efficiency (%) = (10−1/slope − 1).
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Veterinary Research

ISSN: 1297-9716