Table 1 Strains, plasmids, and primers used in this study

Yb2

Riemerella anatipestifer serotype 2 strain

Escherichia coli S17-1

λpirhsdR pro thi; chromosomally integrated RP4-2, Tc::Mu Km::Tn7

Yb2ΔgldM

Tn4351 insertion mutant of R. anatipestifer Yb2, gldM::Tn

cYb2ΔgldM

Mutant Yb2ΔgldM carrying plasmid pCP29-gldM

pCP29

ColE1 ori (pCP1 ori), Ap^r (Em^r); E. coli–F. johnsoniae shuttle plasmid

pCP29-gldM

pCP29 containing ompA promoter and gldM ORF, cfxA(Ap^r)

Primers

 AS87_RS08465-F

5′-ATGGCAAAGGAAAAATT-3′

 AS87_RS08465-R

5′-CTGAACATTTATCACTACTGGAG-3′

 ompA promoter P1

5′-CAGGTACCAGCTAAAATTTTGGCAGTAAC-3′ (KpnI site underlined)

 ompA promoter P2

5′-CGACTCGAGCATTCCAATTCTCTTATTATC-3′ (XhoI site underlined)

 gldM-orf-F

5′-CAGGTACCATGGCAAAGGAAAAATT-3′ (KpnI site underlined)

 gldM-orf-R

5′-CGACTCGAGCTGAACATTTATCACTACTGGA-3′ (XhoI site underlined)

 gldM-pro-F

5′-CGGGATCCATTATACGCTCTTACAATGATAC-3′ (BamHI site underlined)

 gldM-pro-R

5′-GCGTCGACCTGAACATTTATCACTACTGGAG-3′ (SalI site underlined)

 RA ldh-F

5′-ATGAATTATTTTAAACTGCT-3′

 RA ldh-R

5′-TTAGTCTAATTTCTGTATAT-3′

 RA 16S rRNA-F

5′-TCTAAAATGAGATGTTCCA-3′

 RA 16S rRNA-R

5′-ACGAAAGCGTGGGGAGTGG-3′

 Tn4351-F

5′-TGGCACCTTTGTGGTTCTTAC-3′

 Tn4351-R

5′-GAGAGACAATGTCCCCCTTTC-3′