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Table 1 Strains, plasmids, and primers used in this study

From: Riemerella anatipestifer GldM is required for bacterial gliding motility, protein secretion, and virulence

Strains, plasmids, primers Characteristics
Yb2 Riemerella anatipestifer serotype 2 strain
Escherichia coli S17-1 λpirhsdR pro thi; chromosomally integrated RP4-2, Tc::Mu Km::Tn7
Yb2ΔgldM Tn4351 insertion mutant of R. anatipestifer Yb2, gldM::Tn
cYb2ΔgldM Mutant Yb2ΔgldM carrying plasmid pCP29-gldM
pCP29 ColE1 ori (pCP1 ori), Apr (Emr); E. coliF. johnsoniae shuttle plasmid
pCP29-gldM pCP29 containing ompA promoter and gldM ORF, cfxA(Apr)
Primers
 AS87_RS08465-F 5′-ATGGCAAAGGAAAAATT-3′
 AS87_RS08465-R 5′-CTGAACATTTATCACTACTGGAG-3′
 ompA promoter P1 5′-CAGGTACCAGCTAAAATTTTGGCAGTAAC-3′ (KpnI site underlined)
 ompA promoter P2 5′-CGACTCGAGCATTCCAATTCTCTTATTATC-3′ (XhoI site underlined)
 gldM-orf-F 5′-CAGGTACCATGGCAAAGGAAAAATT-3′ (KpnI site underlined)
 gldM-orf-R 5′-CGACTCGAGCTGAACATTTATCACTACTGGA-3′ (XhoI site underlined)
 gldM-pro-F 5′-CGGGATCCATTATACGCTCTTACAATGATAC-3′ (BamHI site underlined)
 gldM-pro-R 5′-GCGTCGACCTGAACATTTATCACTACTGGAG-3′ (SalI site underlined)
 RA ldh-F 5′-ATGAATTATTTTAAACTGCT-3′
 RA ldh-R 5′-TTAGTCTAATTTCTGTATAT-3′
 RA 16S rRNA-F 5′-TCTAAAATGAGATGTTCCA-3′
 RA 16S rRNA-R 5′-ACGAAAGCGTGGGGAGTGG-3′
 Tn4351-F 5′-TGGCACCTTTGTGGTTCTTAC-3′
 Tn4351-R 5′-GAGAGACAATGTCCCCCTTTC-3′
  1. ORF: open reading frame, cfxA: cefoxitin-resistance gene.