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Figure 2 | Veterinary Research

Figure 2

From: Riemerella anatipestifer GldM is required for bacterial gliding motility, protein secretion, and virulence

Figure 2

Expression and identification of R. anatipestifer GldM. A SDS-PAGE analysis of recombinant GldM (rGldM) expression. Lane M: PageRuler Prestained Protein Ladder (Thermo Scientific, Waltham, MA, USA); lane 1: E. coli BL21(DE3) transformed with pET-30a(+), with IPTG induction; lane 2: E. coli BL21(DE3) transformed with pET-gldM, with IPTG induction; lane 3: supernatant from E. coli BL21(DE3) transformed with pET-gldM, with IPTG induction; lane 4: purified rGldM. B Identification of the complementation strain cYb2gldM using a Western blotting assay. Lane 1: whole-cell proteins from the wild-type strain Yb2; lane 2: whole-cell proteins from the mutant strain Yb2ΔgldM; lane 3: whole-cell proteins from the complementation strain cYb2ΔgldM. C Subcellular localization of GldM in R. anatipestifer using a Western blotting assay. Lane 1: whole-cell proteins from the wild-type strain Yb2; lane 2: extract of cytoplasmic proteins from Yb2; lane 3: extract of membrane proteins from Yb2. A second antibody directed against the TonB-dependent receptor of R. anatipestifer was used to control for protein loading in both assays.

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