Table 2 Primers used in this study
Primers | Sequence (5′ to 3′)a | Target genes |
|---|---|---|
For gene expression, deletion and complementation | ||
 cpxREx-F | ACGGGATCCATGAATAAAATCCTGTTAGTTGATGA | cpxR |
 cpxREx-R | ACCAAGCTTTGAAGCAGAAACCATCAGATAG | cpxR |
 cpxRMu-F | GGATTAGCGACGTCTGATGACGTAATTTCTGCCTCGGAGGTATTTAAACAGTGTAGGCTGGAGCTGCTTC | Upstream region of cpxR |
 cpxRMu-R | AAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCATATGAATATCCTCCTTAG | Downstream region of cpxR |
 cpxR-F | TTGATCTTCTGGACGACAGCA | cpxR |
 cpxR-R | CTCAGTACCGGTTAACTCCAGT | cpxR |
 cpxRCo-F | TCCGGATCCATATCAATAATTTCTTGCCGTTC | Upstream region of cpxR |
 cpxRCo-R | CCCAAGCTTGGCCTGACCAATAAAGTTACG | Downstream region of cpxR |
 nlpE-F | GAGCTCGAGGGTGAAAAAAGCGATAGTGA | nlpE |
 nlpE-R | TTCGAATTCTGCCCCAAACTACTGCAATC | nlpE |
For RT-qPCR | ||
 dnaE RT-F | ATGTCGGAGGCGTAAGGCT | dnaE |
 dnaE RT-R | TCCAGGGCGTCAGTAAACAA | dnaE |
 hcp2B RT-F | GTGAAATGCTGCCGAAAGTG | hcp2B |
 hcp2B RT-R | ACAATCGTCGCGTCAGTAAG | hcp2B |
 vgrG RT-F | CGAAGACGCAGATGACGATAC | vgrG |
 vgrG RT-R | GCGTGGATATAGACCTGTTCAC | vgrG |
 xmtU RT-F | GGTGTCATATCCGGTACATCTC | xmtU |
 xmtU RT-R | CTGAACCATGATAAGCAACAGG | xmtU |
 vipA RT-F | TAACACGCCGTTGGATGAG | vipA |
 vipA RT-R | GTTCAGCCGGAACAACAAAC | vipA |
 clpV RT-F | GAGACGCTCGCTACCATTATT | clpV |
 clpV RT-R | TGATTTCGTCCGTCACTTCC | clpV |
 hcp2A RT-F | ACGAAACCGGTGGACAAA | hcp2A |
 hcp2A RT-R | GGTTGGTGCGGTAGAATACA | hcp2A |
For lacZ fusion and EMSA | ||
 Phcp2B-F | TCTAAGCTTAGCTTATGTAATCGTGTTCTGAA | Upstream region of hcp2B |
 Phcp2B-R | GACGGATCCTTGAAATGTAACATGGGGTTGG | hcp2B |
 Phcp2BEMSA-F | AGCTTATGTAATCGTGTTCTG | Upstream region of hcp2B |
 Phcp2B EMSA-R | CATGGGGTTGGCATTTATGAA | hcp2B |
 Phcp2BdeletionEMSA-F | TTGACTAAAAATATATTTAAAC | Upstream region of hcp2B |