Table 1 Primers used to construct the recombinant plasmids
Gene | Accession number | Purpose | Sequence (5′–3′) |
|---|---|---|---|
TGEV-S1 | DQ811786.2 | Construction of pGBKT7-S1 | F: CTCTTCCAGCCCTCCTTCC |
R: GGTCCTTGCGGATGTCG | |||
TGEV-S1-t | XM_003353824.1 | Construction of pGEX-4T-S1 | F: CGGAATTCGAGAAGGCTCTGGCCCTCA |
R: GGCGCATTTCGTCTTCCTGG | |||
UBXN1-t | DQ811786.2 | Construction of pET-28a-UBXN1 | F: TCGGTCGACTCAACTGGGACA-CTTCTT |
R: GCGGTCGACTTAGTTTGTCTAATAATA |