Figure 5

UBXN1 mediated the infection and replication of TGEV. A, B Generation of the viral proliferation curves in IPEC-J2 cells under different conditions. The cells were transfected with siRNA interference fragments and overexpression plasmids for 24 h and were then infected with virus to measure the TCID₅₀ and copy numbers. C, D Quantitative real-time reverse transcription PCR results. At 12 h after the cells were incubated with TGEV Miller, qRT-PCR was performed to measure the mRNA expression levels of TGEV N, UBXN1 and β-actin. The standard curve based on N was used to examine the TGEV copy number. TGEV-infection: virus-infected group; siRNA: virus-infected siRNA interference group. Overexpression: virus-infected overexpression group. NC: blank group. E–G Western blotting results after siRNA interference and viral infection. At 24 h after siRNA interference, the cells were incubated with TGEV Miller. After 48 h, the cells were harvested and assessed by Western blotting for UBXN1, S1, and M protein expression. TGEV: virus-infected group; siRNA TGEV: virus-infected siRNA interference group.