Skip to main content

Advertisement

Table 2 Mean relative gene expression results ± SEM

From: Evidence that the stress hormone cortisol regulates biofilm formation differently among Flavobacterium columnare isolates

Gene Isolate CT Mean ± SEM Mean ± SEM Mean ± SEM500 p-value p-value p-value p-value
    0 50   0 vs 50 0 vs 500 PC vs BC P500 vs B500
gldK LV P 7.2 ± 1.3 4.6 ± 0.9 2.8 ± 0.5 0.03* 0.004** 0.004** 0.04*
B 4.3 ± 0.5 3.9 ± 0.8 3.3 ± 0.6 NS NS   
HV P 12.3 ± 1.9 12.5 ± 2.0 8.2 ± 2.2 NS NS NS NS
B 9.1 ± 2.3 11.3 ± 2.3 9.9 ± 1.0 NS NS   
gldL LV P 58.5 ± 6.5 36.9 ± 5.5 24.9 ± 2.0 0.03* 0.004** 0.004** NS
B 33.3 ± 3.5 31.2 ± 5.3 27.6 ± 3.3 NS NS   
HV P 75.8 ± 7.8 94.8 ± 10.7 68.2 ± 16.1 NS NS 0.03* NS
B 20.4 ± 8.8 56.7 ± 10.0 46.8 ± 9.6 NS NS   
gldM LV P 31.4 ± 5.7 21.0 ± 4.0 15.5 ± 1.5 NS 0.004** 0.03* NS
B 25.9 ± 6.5 21.4 ± 6.0 15.0 ± 1.0 NS NS   
HV P 192.3 ± 23.7 283.2 ± 68.0 248.0 ± 40.5 NS NS 0.047* NS
B 59.8 ± 31.9 204.6 ± 37.6 116.1 ± 20.9 NS NS   
gldN LV P 12.0 ± 1.5 8.8 ± 1.2 6.0 ± 0.6 NS 0.008** 0.02* 0.008**
B 6.8 ± 0.9 5.7 ± 0.9 6.9 ± 0.8 NS NS   
HV P 502.2 ± 66.9 552.9 ± 79.6 407.2 ± 89.6 NS NS NS NS
B 268.8 ± 131.3 313.5 ± 59.1 258.2 ± 59.0 NS NS   
sprA LV P 4.2 ± 1.3 3.9 ± 0.9 5.1 ± 1.4 NS NS 0.004** NS
B 17.7 ± 6.1 22.6 ± 7.9 18.0 ± 6.5 NS NS   
HV P 3.5 ± 0.9 3.4 ± 0.3 4.0 ± 0.6 NS NS 0.03* 0.03*
B 25.3 ± 4.3 23.3 ± 5.9 18.7 ± 3.5 NS NS   
sprE LV P 2.1 ± 0.1 1.8 ± 0.2 2.3 ± 0.4 0.008** NS NS NS
B 3.8 ± 0.7 2.8 ± 0.4 4.2 ± 0.9 NS NS   
HV P 98.6 ± 18.3 96.0 ± 13.4 124.0 ± 21.6 NS NS 0.03* NS
B 406.6 ± 159.4 149.3 ± 18.4 150.2 ± 51.0 NS NS   
sprT LV P 2.6 ± 0.4 3.1 ± 0.1 3.4 ± 0.6 NS NS NS NS
B 3.2 ± 0.5 3.3 ± 0.5 5.0 ± 0.6 NS 0.04*   
HV P 13.9 ± 2.2 13.2 ± 1.4 19.4 ± 1.2 NS NS NS 0.04*
B 37.1 ± 19.2 11.3 ± 2.2 10.0 ± 2.7 NS NS   
porV LV P 6.6 ± 0.7 6.0 ± 0.5 4.2 ± 0.4 NS 0.008** NS 0.008**
B 5.8 ± 0.7 5.5 ± 0.9 6.1 ± 0.6 NS NS   
HV P 70.6 ± 12.7 80.1 ± 10.3 94.6 ± 17.9 NS NS NS NS
B 607.2 ± 364.5 63.4 ± 14.7 47.5 ± 15.0 NS NS   
  1. These include planktonic (P) and biofilm (B) cells of the highly (HV) and low (LV) virulent F. columnare isolate to which a cortisol dose of 0 (control), 50 (LD) or 500 µg/L (HD) was added.
  2. 0, 50 or 500: added cortisol concentration in µg/L; Mean = mean relative gene expression level; SEM: standard error of means; PC vs BC: gene expression in the planktonic cells of the control group compared to the gene expression in the biofilm cells of the control group; P500 vs B500: gene expression in the planktonic cells treated with 500 µg/L cortisol compared to the gene expression in the biofilm cells treated with 500 µg/L cortisol; significantly different p-values are marked in italics with one asterisk indicating p-values < 0.05 and two asterisks p-values < 0.01; p-values depicted are the exact p-values obtained after Wilcoxon matched-pairs signed rank test. NS: not significant.