Evidence that the stress hormone cortisol regulates biofilm formation differently among Flavobacterium columnare isolates

Declercq, Annelies Maria; Cai, Wenlong; Naranjo, Eber; Thongda, Wilawan; Eeckhaut, Venessa; Bauwens, Eva; Arias, Covadonga; De La Fuente, Leonardo; Beck, Benjamin H.; Lange, Miles D.; Peatman, Eric; Haesebrouck, Freddy; Aerts, Johan; Decostere, Annemie

doi:10.1186/s13567-019-0641-3

Veterinary Research

Table 2 Mean relative gene expression results ± SEM

From: Evidence that the stress hormone cortisol regulates biofilm formation differently among Flavobacterium columnare isolates

Gene	Isolate	CT	Mean ± SEM	Mean ± SEM	Mean ± SEM500	p-value	p-value	p-value	p-value
			0	50		0 vs 50	0 vs 500	P_C vs B_C	P₅₀₀ vs B₅₀₀
gldK	LV	P	7.2 ± 1.3	4.6 ± 0.9	2.8 ± 0.5	0.03*	0.004**	0.004**	0.04*
	LV	B	4.3 ± 0.5	3.9 ± 0.8	3.3 ± 0.6	NS	NS
	HV	P	12.3 ± 1.9	12.5 ± 2.0	8.2 ± 2.2	NS	NS	NS	NS
	HV	B	9.1 ± 2.3	11.3 ± 2.3	9.9 ± 1.0	NS	NS
gldL	LV	P	58.5 ± 6.5	36.9 ± 5.5	24.9 ± 2.0	0.03*	0.004**	0.004**	NS
	LV	B	33.3 ± 3.5	31.2 ± 5.3	27.6 ± 3.3	NS	NS
	HV	P	75.8 ± 7.8	94.8 ± 10.7	68.2 ± 16.1	NS	NS	0.03*	NS
	HV	B	20.4 ± 8.8	56.7 ± 10.0	46.8 ± 9.6	NS	NS
gldM	LV	P	31.4 ± 5.7	21.0 ± 4.0	15.5 ± 1.5	NS	0.004**	0.03*	NS
	LV	B	25.9 ± 6.5	21.4 ± 6.0	15.0 ± 1.0	NS	NS
	HV	P	192.3 ± 23.7	283.2 ± 68.0	248.0 ± 40.5	NS	NS	0.047*	NS
	HV	B	59.8 ± 31.9	204.6 ± 37.6	116.1 ± 20.9	NS	NS
gldN	LV	P	12.0 ± 1.5	8.8 ± 1.2	6.0 ± 0.6	NS	0.008**	0.02*	0.008**
	LV	B	6.8 ± 0.9	5.7 ± 0.9	6.9 ± 0.8	NS	NS
	HV	P	502.2 ± 66.9	552.9 ± 79.6	407.2 ± 89.6	NS	NS	NS	NS
	HV	B	268.8 ± 131.3	313.5 ± 59.1	258.2 ± 59.0	NS	NS
sprA	LV	P	4.2 ± 1.3	3.9 ± 0.9	5.1 ± 1.4	NS	NS	0.004**	NS
	LV	B	17.7 ± 6.1	22.6 ± 7.9	18.0 ± 6.5	NS	NS
	HV	P	3.5 ± 0.9	3.4 ± 0.3	4.0 ± 0.6	NS	NS	0.03*	0.03*
	HV	B	25.3 ± 4.3	23.3 ± 5.9	18.7 ± 3.5	NS	NS
sprE	LV	P	2.1 ± 0.1	1.8 ± 0.2	2.3 ± 0.4	0.008**	NS	NS	NS
	LV	B	3.8 ± 0.7	2.8 ± 0.4	4.2 ± 0.9	NS	NS
	HV	P	98.6 ± 18.3	96.0 ± 13.4	124.0 ± 21.6	NS	NS	0.03*	NS
	HV	B	406.6 ± 159.4	149.3 ± 18.4	150.2 ± 51.0	NS	NS
sprT	LV	P	2.6 ± 0.4	3.1 ± 0.1	3.4 ± 0.6	NS	NS	NS	NS
	LV	B	3.2 ± 0.5	3.3 ± 0.5	5.0 ± 0.6	NS	0.04*
	HV	P	13.9 ± 2.2	13.2 ± 1.4	19.4 ± 1.2	NS	NS	NS	0.04*
	HV	B	37.1 ± 19.2	11.3 ± 2.2	10.0 ± 2.7	NS	NS
porV	LV	P	6.6 ± 0.7	6.0 ± 0.5	4.2 ± 0.4	NS	0.008**	NS	0.008**
	LV	B	5.8 ± 0.7	5.5 ± 0.9	6.1 ± 0.6	NS	NS
	HV	P	70.6 ± 12.7	80.1 ± 10.3	94.6 ± 17.9	NS	NS	NS	NS
	HV	B	607.2 ± 364.5	63.4 ± 14.7	47.5 ± 15.0	NS	NS

These include planktonic (P) and biofilm (B) cells of the highly (HV) and low (LV) virulent F. columnare isolate to which a cortisol dose of 0 (control), 50 (LD) or 500 µg/L (HD) was added.
0, 50 or 500: added cortisol concentration in µg/L; Mean = mean relative gene expression level; SEM: standard error of means; P_C vs B_C: gene expression in the planktonic cells of the control group compared to the gene expression in the biofilm cells of the control group; P₅₀₀ vs B₅₀₀: gene expression in the planktonic cells treated with 500 µg/L cortisol compared to the gene expression in the biofilm cells treated with 500 µg/L cortisol; significantly different p-values are marked in italics with one asterisk indicating p-values < 0.05 and two asterisks p-values < 0.01; p-values depicted are the exact p-values obtained after Wilcoxon matched-pairs signed rank test. NS: not significant.

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ISSN: 1297-9716

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