Dectin-2 is expressed in ovine ruminal mucosa tissue and OREC. A Dectin-2 expression in the spinous layer cells and basement layer cells of ovine ruminal mucosa tissue assessed by immunohistochemistry. Formalin-fixed paraffin-embedded sections of mucosa from the ovine rumen were stained with Dectin-2 Mouse mAb at 10 μg/mL. (Bottom panels) Staining with isotype-matched control antibodies served as a negative control. The picture on the right (40×) is an enlarged view of the red frame on the left side (10×). B Dectin-2 PCR results. Marker: D2000 DNA Marker; D-2: Dectin-2; N: Negative control. C Western blotting analysis of OREC cell lysates showing the Dectin-2 band (35 kDa). Blots were reacted with Dectin-2 Mouse mAb and with β-actin Mouse mAb as loading controls, and the four lanes in the figure represent four repetitions of the same lane.