Figure 1
From: Low levels of classical BSE infectivity in rendered fat tissue
Processing of the inoculum. A Fat was rendered from the Celiac and mesenteric ganglion complex samples (arrow) which were embedded in mesentery fat (asterisk); B samples were incubated for 20 min at 95 °C according to standard tallow production methods; C the melted fat was taken, but still contained tissues remnants; D 100 µL of the liquid fat was 1:5 diluted in physiological saline, thoroughly vortexed and cleaned by a short centrifugation at 10 000 rpm. The resulting supernatant was taken as inoculum.