Figure 9
rFBA and fibronectin interaction analysis by Far-WB and Surface plasmon resonance (SPR) analysis. A Far-WB analysis of rFBA with fibronectin. The first lane: PVDF membrane with transferred rFBA protein incubated with anti- rFBA antibody as a positive control; the second lane: PVDF membrane with transferred rFBA protein incubated with fibronectin and anti-fibronectin antibody; the third lane: PVDF membrane with transferred BSA (negative control) incubated with fibronectin and anti-fibronectin antibody. Protein bands were visualized using ECL substrate. B Sensorgrams depict the binding of immobilised fibronectin to rFBA. Increasing concentrations of rFBA (5, 10, 25, 50 and 100 μg/mL) were injected at a flow rate of 30 μL/min for 180 s over immobilised fibronectin. The arrow indicates the end of the injection period, at which point dissociation of rFBA from fibronectin can be observed. RU resonance units.