Figure 5

Western blot analysis of comparative proteomics data. A The left lane was loaded with bacterial proteins from M. hyopneumoniae strain 168. The right lane was loaded with bacterial proteins from strain 168L. The differentially abundant protein fructose-1,6-bisphosphate aldolase (FBA; 44 kDa) was analysed using the corresponding antibodies. Protein bands were visualised using Electro-Chemi-Luminescence (ECL) substrate. A Ponceau-S stained membrane was used as the loading control. B Image J software was used to calculate the optical density of the corresponding bands in the blots. The optical density of the corresponding bands was normalized to the total proteins of Ponceau-S staining of the same membrane. The level of abundance of FBA in M. hyopneumoniae strain 168L is expressed as the percentage of that in M. hyopneumoniae strain 168. The asterisk above the charts stands for statistically significant differences.