Skip to main content

Table 1 Bacterial strains and plasmids used in this study

From: Characterization of the zinc metalloprotease of Streptococcus suis serotype 2

Strains/plasmids General characteristics Source/references
Escherichia coli
 TOP 10 F mrcA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (StrR) endA1 nupG Invitrogen
 BL21 FompT hsdSB (r B , m B ) gal dcm rne131 (DE3) Invitrogen
Streptococcus suis
 P1/7 Wild-type, virulent European serotype 2 ST1 strain isolated from pig with meningitis [56]
 Δzmp Isogenic mutant strain derived from P1/7; in frame deletion of zmp gene This work
 compΔzmp Mutant Δzmp complemented with pMXzmp complementation vector This work
 SC84 Highly virulent clonal serotype 2 ST7 strain isolated from the 2005 human outbreak in China [57]
Streptococcus pneumoniae
 TIGR4 Virulent serotype 4 strain isolated from human blood in Norway ATCC
Plasmids
 PCR2.1 Apr, Kmr, oriR(f1) MCS oriR (ColE1) Invitrogen
 pSET4s Thermosensitive vector for allelic replacement in S. suis. Replication functions of pG + host3, MCS oriR pUC19 lacZ SpR [20]
 pMX1 Replication functions of pSSU1, MCS pUC19 lacZ SpR, malX promoter of S. suis, derivative of pSET2 [20, 58]
 pET101 Apr, pBR322 ori, T7 promotor Invitrogen
 p4Δzmp pSET4 s carrying the construct of zmp for allelic replacement This work
 pMXzmp pMX1 complementation vector carrying intact zmp gene This work
 pET101zmp pET101 carrying zmp gene for protein production This work