Figure 2
From: Safety and immunogenicity of plant-produced African horse sickness virus-like particles in horses
Western blot analysis of pre- and post- (day 41) vaccination antisera. Pre- and post- (day 41) vaccination antisera (1:5000 dilution) from horse 1 (lanes 2 and 3), horse 2 (lanes 4 and 5), horse 3 (lane 6 and 7) and horse 4 (lanes 8 and 9) were used to detect AHS 5 VLP proteins in a standard Western blot analysis. No AHSV proteins were detected in any of the pre-vaccination sera (PB) (lanes 2, 4, 6 and 8) but all four were detected in serum sampled at days 41 post-vaccination (D41) (lanes 3, 5, 7 and 9). Protein bands of AHS viral proteins VP2, VP3, VP5 and VP7 are indicated with arrows; colour pre-stained protein molecular weight marker (New England Biolabs, Massachusetts, USA) is in lane 1.