Figure 3
SG-based M2eCD40L vaccine elicits M2e and SG specific systemic IgG and mucosal IgA responses. The serum IgG (1:100 dilution) and mucosal IgA (1:30 dilution) responses in intestinal and lung wash samples were analysed by an indirect ELISA. The antibody responses were measured at 28th day post-vaccination. A M2e-specific serum IgG responses. B M2e-specific intestinal sIgA responses. C M2e-specific lung sIgA responses. D ompA-specific serum IgG responses. E ompA-specific intestinal sIgA responses. Each data points represent mean ± SD of five chickens per group for IgG analysis and three chickens for IgA analysis. Statistical significance was determined by one-way ANOVA (with Tukey’s multiple comparisons tests). *p < 0.05. ns: non-significant.