Figure 5

Caveolin-1 is involved in arginine uptake during TGEV infection. A Western blot analysis was performed to determine the levels of caveolin-1 protein 48 h after TGEV infection. B Cells were transfected with three caveolin-1 specific shRNAs or the shRNA control. Cell extracts were then subjected to Western blot analysis to measure expression of caveolin-1 and CAT-1. C The uptake of arginine was assayed. D Cells were transfected with three CAT-1 specific shRNAs or the shRNA control. Western blot analysis was used to measure expression of CAT-1 and caveolin-1. E Cells were transfected with a plvx-CAT-1 shRNA or the plvx control. Western blot analysis was used to measure expression of CAT-1 and caveolin-1 after TGEV infection. Differences were considered significant at *0.01 < p < 0.05, **p < 0.01.