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Table 1 Effects and dosages of chemicals used in this study

From: Porcine sapovirus Cowden strain enters LLC-PK cells via clathrin- and cholesterol-dependent endocytosis with the requirement of dynamin II

Chemicals Effects Dosages Solvent
LLC-PK cells Caco-2 cells MA104 cells
Chlorpromazine Inhibits clathrin-mediated endocytosis 1 μM, 10 μM, 20 μM 1 μM, 10 μM, 20 μM 1 μM, 10 μM, 20 μM DW
Methyl-ß-cyclodextrin Depletes cholesterol 1 mM, 10 mM, 20 mM 0.5 mM, 1 mM, 5 mM 1 mM, 5 mM, 10 mM DW
Soluble cholesterol Replenish cholesterol 10 μM, 100 μM, 200 μM 10 μM, 50 μM, 100 μM 10 μM, 50 μM, 100 μM DW
Nystatin Inhibits caveolae-mediated endocytosis, sequesters cholesterol 1 μM, 10 μM, 25 μM 1 μM, 10 μM, 25 μM 1 μM, 25 μM, 50 μM DMSO
Dynasore Inhibits dynamin 1 μM, 50 μM, 100 μM 1 μM, 50 μM, 100 μM 1 μM, 50 μM, 100 μM DMSO
Chloroquine Inhibits acidification of endosomes 1 μM, 50 μM, 100 μM DW
Cytochalasin D Disrupts actin cytoskeleton 0.5 μM, 1 μM, 5 μM DMSO
Nocodazole Disrupts microtubules 1 μM, 10 μM, 20 μM DMSO
Amiloride Inhibits macropinocytosis 1 μM, 50 μM, 100 μM DMSO
  1. DW, distilled water; DMSO, dimethyl sulfoxide.