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Table 1 Virus detection in the organs of birds of experiment 1 by means of RRT-PCR, IHC and isolation in MDCK cells

From: A G1-lineage H9N2 virus with oviduct tropism causes chronic pathological changes in the infundibulum and a long-lasting drop in egg production

Day post-infection 4 6 10
RRT-PCR pos/tot (%)a IHC pos/tot (%) TCID50 pos/tot (%) RRT-PCR pos/tot (%) IHC pos/tot (%) TCID50 pos/tot (%) RRT-PCR pos/tot (%) IHC pos/tot (%) TCID50 pos/tot (%)
Oviduct 5/7 (71) 2/7 (29) 3/7 (43) 3/7 (57) 3/7 (43) 3/7 (43) 1/7 (14) 1/7 (14) 0/7 (0)
Intestine 4/4 (100) 0/4 (0) 1/4 (25) 2/4 (50) 0/4 (0) 0/4 (0) 0/4 (0) 0/4 (0) 0/4 (0)
Cecal tonsil 2/4 (50) 0/4 (0) 1/4 (25) 2/4 (50) 0/4 (0) 0/4 (0) 0/4 (0) 0/4 (0) 0/4 (0)
Kidney 1/4 (25) 0/4 (0) 0/4 (0) 2/4 (50) 0/4 (0) 0/4 (0) 0/4 (0) 0/4 (0) 0/4 (0)
Spleen 1/4 (25) 0/4 (0) 0/4 (0) 2/4 (50) 0/4 (0) 0/4 (0) 1/4 (25) 0/4 (0) 0/4 (0)
Lung 3/4 (75) 0/4 (0) 2/4 (50) 3/4 (75) 0/4 (0) 0/4 (0) 3/4 (75) 0/4 (0) 0/4 (0)
Brain 1/4 (25) 0/4 (0) 0/4 (0) 0/4 (0) 0/4 (0) 0/4 (0) 0/4 (0) 0/4 (0) 0/4 (0)
  1. pos: positive, tot: total.
  2. aPercentage of positive animals; oviducts were considered positive when at least one section of the organ tested positive.