A G1-lineage H9N2 virus with oviduct tropism causes chronic pathological changes in the infundibulum and a long-lasting drop in egg production

Table 1 Virus detection in the organs of birds of experiment 1 by means of RRT-PCR, IHC and isolation in MDCK cells

Day post-infection	4			6			10
Day post-infection	RRT-PCR pos/tot (%)^a	IHC pos/tot (%)	TCID₅₀ pos/tot (%)	RRT-PCR pos/tot (%)	IHC pos/tot (%)	TCID₅₀ pos/tot (%)	RRT-PCR pos/tot (%)	IHC pos/tot (%)	TCID₅₀ pos/tot (%)
Oviduct	5/7 (71)	2/7 (29)	3/7 (43)	3/7 (57)	3/7 (43)	3/7 (43)	1/7 (14)	1/7 (14)	0/7 (0)
Intestine	4/4 (100)	0/4 (0)	1/4 (25)	2/4 (50)	0/4 (0)	0/4 (0)	0/4 (0)	0/4 (0)	0/4 (0)
Cecal tonsil	2/4 (50)	0/4 (0)	1/4 (25)	2/4 (50)	0/4 (0)	0/4 (0)	0/4 (0)	0/4 (0)	0/4 (0)
Kidney	1/4 (25)	0/4 (0)	0/4 (0)	2/4 (50)	0/4 (0)	0/4 (0)	0/4 (0)	0/4 (0)	0/4 (0)
Spleen	1/4 (25)	0/4 (0)	0/4 (0)	2/4 (50)	0/4 (0)	0/4 (0)	1/4 (25)	0/4 (0)	0/4 (0)
Lung	3/4 (75)	0/4 (0)	2/4 (50)	3/4 (75)	0/4 (0)	0/4 (0)	3/4 (75)	0/4 (0)	0/4 (0)
Brain	1/4 (25)	0/4 (0)	0/4 (0)	0/4 (0)	0/4 (0)	0/4 (0)	0/4 (0)	0/4 (0)	0/4 (0)

pos: positive, tot: total.
^aPercentage of positive animals; oviducts were considered positive when at least one section of the organ tested positive.

ISSN: 1297-9716