Far Western blot analysis of rTsgal binding to IEC proteins. A SDS-PAGE analysis of the IEC proteins. Lane M: The protein molecular weight marker; lane 1: The IEC proteins; lane 2: The C2C12 lysates. B Far Western analysis of the rTsgal binding to IEC proteins. The IEC proteins blotted on the PVDF membrane was pre-incubated with rTsgal (lane 1–3), PBS (lane 4–6), C2C12 proteins was also pre-incubated with rTsgal (lane 7–9). Subsequently, both cell proteins were incubated with anti-rTsgal serum (lane 1, 4 and 7), infection serum (lane 2, 5 and 8) or normal serum (lane 3, 6 and 9), respectively. The binding between rTsgal and IECs was detected only with anti-rTsgal serum (lane 1) and infection serum (lane 2).