Figure 6
Expression and immunolocalization of Tsgal at T. spiralis various developmental phases. A-E: The results of IFA with intact nematodes probed by anti-rTsgal serum. The intense immunostaining was observed on the surface of ML A, IIL B and NBL E, but slight staining was found on the surface of 3 days male (C) and female (D). The ML (F) recognized by infection serum was served as a positive control; the ML incubated with normal serum (G) and PBS (H) was served as negative control. I–L Immunolocalization of Tsgal in Trichinella spiralis ML and AW using IFA with worm sections. By using anti-Tsgal serum the intense immunostaining was located at the cuticles and stichosomes of the ML (I), and embryos of female adult at 3 dpi (J). The section of ML (K) recognized by infection serum as a positive control; The ML (L) not recognized by normal serum as negative controls. Scale-bars: A, B, F–L, 200 μm; C, D 100 μm; E, 500 μm.