Identification of recombinant Tsgal protein. A SDS-PAGE analysis of the rTsgal. Lane M: The protein molecular weight marker; lane 1: T. spiralis ML somatic proteins; lane 2: ML ES protein; lane 3: the purified rTsgal. B Western blot analysis of the rTsgal antigenicity. ML somatic proteins (lane 1), ML ES proteins (lane 2) and the rTsgal (lane 3) were probed with mouse infection sera. By using anti-Tsgal serum, the natural Tsgal protein was found in ML somatic proteins (lane 4), but not in ML ES proteins (lane 5), and rTsgal (lane 6) was also probed by anti-Tsgal serum. The ML somatic proteins (lane 7), ES proteins (lane 8) and rTsgal (lane 9) were not probed with normal mouse serum. C Western blot analysis indicated that native Tsgal was recognized by anti-Tsgal serum in somatic proteins of all lifecycle stages of T. spiralis (lane 1: ML; lane 2: IIL; lane 3: 3 days AW; lane 4: 6 days AW, lane: NBL).