Table 1 Primers used for expression plasmid construction
Primers | Sequences (5′ to 3′) |
|---|---|
pFU-ANXA2-F | CGCGGATCC ATGTCTACCGTTCATGAAATTCT |
pFU-ANXA2-R | TAACACCGGTTCAGTCATCCCCACCACACAG |
pANXA2-F | TTGGGCCCACCATGTCTACCGTTCATGAAATTCT |
pANXA2-Flag-R | CCGCTCGAGTCACTTATCGTCGTCATCCTTGTAATCGTCATCCCCACCACACAG |
pVIM-F | TTGGGCCCACCATGACCACCAGGTCCGTGTC |
pVIM-HA-R | CCGGAATTCTTAAGCGTAATCTGGAACATCGTATGGGTATTCAAGGTCATCGTGATGC |
ANXA2-Flag(1–102)-F | TTGGGCCCACCATGTCTACCGTTCATGAAATTCT |
ANXA2-Flag(1–102)-R | CCGCTCGAGTCACTTATCGTCGTCATCCTTGTAATCTAGGCCCAAAATCACTGTC |
ANXA2-Flag(1–174)-F | TTGGGCCCACCATGTCTACCGTTCATGAAATTCT |
ANXA2-Flag(1–174)-R | CCGCTCGAGTCACTTATCGTCGTCATCCTTGTAATCCAGGGCAACCATCAGCTTG |
ANXA2-Flag(1–259)-F | TTGGGCCCACCATGTCTACCGTTCATGAAATTCT |
ANXA2-Flag(1–259)-R | CCGCTCGAGTCACTTATCGTCGTCATCCTTGTAATCCAGGTTCAGGAAAGCATTTT |
ANXA2-Flag(109–340)-F | TTGGGCCCACCATGTATGACGCTTCCGAGCTGAAA |
ANXA2-Flag(109–340)-R | CCGCTCGAGTCACTTATCGTCGTCATCCTTGTAATCGTCATCCCCACCACACAG |
ANXA2-Flag(192–340)-F | TTGGGCCCACCATGCAAGATGCCCGGGATCTCTAT |
ANXA2-Flag(192–340)-R | CCGCTCGAGTCACTTATCGTCGTCATCCTTGTAATCGTCATCCCCACCACACAG |