Figure 7

ANXA2/vimentin binding to PRRSV structural proteins. A Validation of the reactivity of PRRSV structural protein mAbs and the PRRSV proteins by SDS-PAGE. B The mixture of PRRSV protein incubated with the extraction of HEK293T transfected with plasmids expressing tagged ANXA2 protein and subjected to Co-IP. The precipitated proteins were performed using anti-GP2, anti-GP3, anti-GP5, anti-M, and anti-N mAbs. C The binding of vimentin to PRRSV structural proteins. The mixture of PRRSV protein incubated with the extraction of HEK293T transfected with plasmids expressing tagged vimentin protein and then subjected to Co-IP. D The binding of the ANXA2-vimentin complex to PRRSV structural proteins. The mixture of PRRSV protein mixed with ANXA2-vimentin complex, and then performed the Co-IP assay. The triangle (black up-pointing triangle) highlights the position of the PRRSV N protein. E The mixture of PRRSV protein mixed with HEK293T transfected with plasmids expressing tagged ANXA2 and vimentin protein or control vector, and then performed Co-IP assay using anti-Flag affinity gel. The precipitated proteins were tested by Western blotting using the corresponding antibodies.