Co-localization of ANXA2 and vimentin in PAMs, Marc-145 cells. PAMs and Marc-145 cells at 70% confluence were washed, fixed, blocked with 5% skimmed milk and then incubated with rabbit anti-ANXA2 mAb (Cell Signaling Technology) or mouse anti-vimentin mAb (Cell Signaling Technology) at 4 °C overnight and then incubated for 1 h with goat anti-rabbit IgG-FITC antibodies or goat anti-mouse IgG-TRITC antibodies. Finally, the cells were then counter-stained with DAPI and subjected to confocal assay. Fluorescent staining of ANXA2 (green) and vimentin (red) in PAMs and Marc-145 cells, counterstained with DAPI (blue). The scale bar indicates 10 µm.