ANXA2 was identified as a differentially upregulated protein by 2D-DIGE/MS analysis. A PAMs were infected with PRRSV HuN4 at a MOI of 0.01. At 48 hpi, cells were harvested and analyzed by 2D-DIGE. The 2D-DIGE profiles of differentially expressed protein levels in either the uninfected control PAM or HuN4-infected PAM. The 3D spot intensity represents the differentially expressed ANXA2 protein. B ANXA2 was identified using Western blotting. PAMs were infected with PRRSV HuN4 at a MOI of 0.01. At 48 hpi, cells were harvested for analysis by Western blotting. C The relative intensity ratios of ANXA2. D Bioinformatics analysis about ANXA2 was conducted using STRING and the interaction network revealed that ANXA2 and other proteins (including vimentin) form a protein interaction network.