Construction strategy and immunofluorescence staining of PK15S10–CD163 cells. A Schematic representation of the construction of the PK15S10–CD163 cell line. To construct a cell line co-expressing Siglec-10 and CD163, PK15 cells were first transfected with a plasmid containing Siglec-10 cDNA and a geneticin resistance gene. The cells were subcloned and screened for Siglec-10 expressing cells. After selection for geneticin resistance, the obtained PK15S10 cells were transfected with a plasmid containing the CD163 cDNA and a zeocin resistance gene, which allowed the selection of cells expressing both Sn and CD163. B Immunofluorescence staining for PK15S10–CD163. The green color represents Siglec-10, while the red color represents CD163. The nuclei of the cells are blue (Hoechst). Scale bar: 25 µm.