ADCC assays of T. spiralis NBL (A–C) and ML (D–F). A, D The cytotoxicity was anti-rTsSP antibody dose-dependent. B, E The cytotoxicity had an increasing trend with prolonged culture time. C, F ADCC assay with different serum. When 1:100 dilutions of different serum was used and cultured for 72 h, the ADCC with anti-rTsSP serum caused the evident death of NBL (C) and ML (F), compared with the larvae incubated by normal mouse serum (pre-immune serum). Asterisks show a statistically prominent difference (P < 0.01) in comparison with normal serum.