Comparison response CD4, CD8 T cells from unvaccinated steers stimulated with MMP-pulsed bDC and MoDC. A Representative flow cytometric profiles showing level of CD4 T cell proliferation following one and two rounds of antigenic stimulation by Ag-pulsed DC. B Representative flow cytometric profiles showing level of CD8 T cell proliferation following one and two rounds of antigenic stimulation by Ag-pulsed DC. C Two-way ANOVA with interaction. The factors were type of T cell (two levels: CD4, CD8) and MMP-stimulation (two levels: control, MMP). Tukey HSD (overall α = 0.05) was applied post-hoc to determine the statistical significance of comparisons of interest. The mean and standard deviation for each treatment effect for each type of T cell is shown (n = 4 unvaccinated steers). The two-way ANOVA was significant (F = 732.6776; P < 0.0001). The mean effect of MMP stimulation was significant (F
= 2188.613, P < 0.0001) but not dependent on T cell type (F
= 1.33; P = 0.2092). The mean effect of T cell type was small but significant (CD4 effect estimate = 1.55 ± 0.56; F
= 7.6591, P = 0.0170).