Detection of Ide
(A) and IgM cleavage products (B) in Western blot analysis. A Anti-Ide
Western blot of 30-fold concentrated culture supernatants of the indicated S. suis strains. B Anti-IgM Western blot of porcine serum incubated with 30-fold concentrated S. suis culture supernatants. The arrow marks the uncleaved IgM heavy chain, asterisks indicate cleavage products of the heavy chain at ~41 and ~32 kDa. The marker bands are shown on the left (sizes in kDa). Western blots were conducted under reducing conditions.