Figure 4
Viral antigen localization in the liver of rabbits (Oryctolagus cuniculus) inoculated with Lagovirus europaeus G1.2 (BlMt-1). Asterisks denote portal areas. A Uninfected control (rabbit 130). No antigen (brown staining) is seen. 200× magnification. B Earlier stage of acute infection in an adult rabbit (rabbit 283). A small number of periportal hepatocytes contain viral antigen (brown staining, arrow). A cluster of heterophils surrounds necrotic, antigen-containing debris interpreted to be the remnants of an infected hepatocyte (arrowhead). 200× magnification. C Terminal stage of acute infection in an adult rabbit (rabbit 282). There is cytoplasmic staining in hepatocytes throughout the lobule (arrows). 200× magnification. D Terminal stage of acute infection in a 5 week old rabbit (rabbit 303). In addition to the finely stippled intracytoplasmic staining of infected hepatocytes, there is occasional intranuclear staining (arrow, arrowhead) and staining of the cell membrane (arrowhead). 400× magnification.