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Figure 4 | Veterinary Research

Figure 4

From: Atrophy of primary lymphoid organs induced by Marek’s disease virus during early infection is associated with increased apoptosis, inhibition of cell proliferation and a severe B-lymphopenia

Figure 4

Cells expressing MDV antigens are apoptotic in the thymus and rarely in the bursa. Cryosections of thymus (A, C, E) and bursa (B, D, F) were stained for MDV antigens (lytic: VP22, ICP4, gB and/or latent: Meq) (green) and apoptosis was detected by TUNEL assay (red). DNA content of the cells were stained with Hoescht 33342 dye (white). MDV antigens positive cells and apoptotic cells were detected in both organs at 6 dpi (A, B), 10 dpi (C, D) and 14 dpi (E, F). At 6 dpi, in the thymus, the majority of the cells expressing the lytic MDV-antigens were apoptotic, in contrast to the bursa. At 6 dpi, in the bursa, only rare double positive cells were detected (as shown in panel B, in the enlarged frame). In both organs, at 10 and 14 dpi, no co-localization between MDV antigens (including Meq) and TUNEL signal was observed. Bar, 20 µm

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Veterinary Research

ISSN: 1297-9716