Figure 1
Generation of anti-S. aureus-specific bovine IgG antibodies. S. aureus, S. epidermidis, B. atrophaeus, and E. coli were used to confirm the specificity of antibodies against S. aureus (anti-S. aureus) generated by immunization of a Holstein dairy cow with killed S. aureus. A Genetic similarity of the four species of bacteria used in this study and other well-characterized bacteria is shown as a phylogenetic tree. B ELISA analyses show that anti-S. aureus was highly reactive with two strains of S. aureus (BM1006 and SA003) and less reactive S. epidermidis. In contrast, little reactivity was detected when anti-S. aureus was reacted with B. atrophaeus and E. coli. C Flow cytometric analyses detected similar reactivities, respectively. D SDS-PAGE and western blot analyses revealed that anti-S. aureus reacted with S. aureus-specific molecules with a broad range of molecular masses. M: molecular marker, 1: S. aureus (BM1006), 2: S. aureus (SA003), 3: S. epidermidis, 4: B. atrophaeus, 5: E. coli. Three separate experiments were performed, and the data represent the mean ± standard error of the mean. *p < 0.05.