Table 2 Genes analysed by RT-qPCR. Length of the PCR fragments amplified, and primers used

TLRs

TLR2^a

ENSSSCG00000009002

TLR2-F 5′-TCACTTGTCTAACTTATCATCCTCTTG-3′

TLR2-R 5′-TCAGCGAAGGTGTCATTATTGC-3′

TLR3^a

ENSSSCG00000015801

TLR3-F 5′-AGTAAATGAATCACCCTGCCTAGCA-3′

TLR3-R 5′-GCCGTTGACAAAACACATAAGGACT-3′

TLR4^a

ENSSSCG00000005503

TLR4-F 5′-GCCATCGCTGCTAACATCATC-3′

TLR4-R 5′-CTCATACTCAAAGATACACCATCGG-3′

TLR5^a

Gene ID 100144476

TLR5-F 5′-CTCGCCCACCACATTA-3′

TLR5-R 5′-TGAGGGTCCCAAAGAGT-3′

TLR7^a

ENSSSCT00000035232.2

TLR7-F 5′-GGGAAAGCTCCAGTATCTGC-3′

TLR7-R 5′-TGAGGCTTCTGGAACAGTTG-3′

TLR8^a

ENSSSCG00000012118

TLR8-F 5′-AAGACCACCACCAACTTAGCC-3′

TLR8-R 5′-GACCCTCAGATTCTCATCCATCC-3′

TLR9^a

ENSSSCG00000011436

TLR9-F 5′-CACGACAGCCGAATAGCAC-3′

TLR9-R 5′-GGGAACAGGGAGCAGAGC-3′

TNF-α^a

ENSSSCG00000001404

TNF-α-F 5′-TGGTGGTGCCGACAGATGG-3′

IFN-γ^a

ENSSSCT00000055560.1

TNFG-F-5′-CTGGGAAACTGAATGACTTCG-3′

TNFG-R-5′-TCTGACTTCTCTTCCGCTTTC-3′