Figure 3

The character of CVP1 peptide cell-penetrating activity. A Analysis of the intake of CVP1 peptide in HCT116 cells at increasing concentration by flow cytometry. HCT116 cells were treated with different concentrations (1, 2, 5, 10, 20, 40 µM) of CVP1 or Mut-CVP1 peptide for 30 min at 37 °C. The data was shown using the FlowJo software. B HCT116 cells treated with the CVP1 peptide for the different incubation time points (5, 10, 15, 20, 25, 30 min) at a peptide concentration of 5 µM. C Percentage of viable HCT116 cells after treatment with CVP1 peptide at various concentrations (5, 10, 20, 40 µM) for 12, 24, and 48 h. D The cellular uptake efficiency of CVP1 and TAT peptide were evaluated by flow cytometry at 0.1, 1, 2, 5 and 10 µM. Single asterisk (*) and double asterisks (**) indicated P < 0.05 and P < 0.01, respectively.