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Figure 4 | Veterinary Research

Figure 4

From: Oral immunization with a novel attenuated Salmonella Typhimurium encoding influenza HA, M2e and NA antigens protects chickens against H7N9 infection

Figure 4

qRT-PCR analysis of cytokine gene expressions in PBMCs after stimulation with inactivated H7N9 antigen. Chickens (N = 12) were immunized with Sal-vector, Sal-HA, Sal-M2e, Sal-M2e-NA or Sal-HA-M2e-NA vaccine, and PBMCs from immunized chickens after 14 days post-immunization were stimulated with inactivated H7N9 antigen for 24 h and analysed for induction of IFN-ϒ (A), IL-17 (B) and IL-10 (C) mRNA transcription levels by qRT-PCR assay. Results are expressed as fold change in mRNA transcription after inactivated H7N9 antigen stimulation of immunized PBMCs compared to the inactivated H7N9 treated naïve PBMCs. Gene expressions were normalized to GAPDH and mRNA levels of naive stimulated cells were used as calibrator. Data presented are mean ± SD of five chickens per group. *p < 0.05. ns: non-significant.

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Veterinary Research

ISSN: 1297-9716