Cytokines levels in ten mice immunized with
Choleraesuis vaccines. IFN-γ (A), IL-4 (B) or IL-17A (C) in sera at 0.5, 3, and 5 days, in the spleen at 7 days after the boost were assayed with ELISA kit. BSG control was also included. *P < 0.05; **P < 0.01, for the cytokines levels induced by strains rSC0011, rSC0016 and rSC0018 containing pS-SaoA compared with that containing the empty vector pYA3493; #
P < 0.05, ##
P < 0.01 for the significant differences between groups were indicated. The assay was performed in triplicate. The data were collected from two experiments and analyzed.