Peptide inhibition assay. Subadult L. vannamei shrimp were randomly divided into five groups (5 individuals per group). For the negative-control group, each shrimp was infused with 30 μL of normal saline. For the WSSV-CN03 infected groups, the shrimp were inoculated orally with 2 × 109 WSSV-CN03 virions plus 120 μg of P-VP24186–200 or P-VP24148–162. For the WSSV-CN04 infected groups, the shrimp were inoculated orally with 2 × 109 WSSV-CN04 virions plus 120 μg of P-VP24186–200 or P-VP24148–162. The tissues were collected from each group at 4 hpi for qPCR analysis (A, B). The data were subjected to independent-sample t test as indicated in figure. *P < 0.05; **P < 0.01. A section of midgut was used for cryosectioning (C) and probed with anti-VP28 antibody (green). The nucleus was stained with DAPI (blue). Bar 100 μm. The experiment was repeated three times and representative results from one experiment are shown.