Figure 7
From: A VP24-truncated isolate of white spot syndrome virus is inefficient in per os infection
Peptide inhibition assay. Subadult L. vannamei shrimp were randomly divided into five groups (5 individuals per group). For the negative-control group, each shrimp was infused with 30 μL of normal saline. For the WSSV-CN03 infected groups, the shrimp were inoculated orally with 2 × 109 WSSV-CN03 virions plus 120 μg of P-VP24186–200 or P-VP24148–162. For the WSSV-CN04 infected groups, the shrimp were inoculated orally with 2 × 109 WSSV-CN04 virions plus 120 μg of P-VP24186–200 or P-VP24148–162. The tissues were collected from each group at 4 hpi for qPCR analysis (A, B). The data were subjected to independent-sample t test as indicated in figure. *P < 0.05; **P < 0.01. A section of midgut was used for cryosectioning (C) and probed with anti-VP28 antibody (green). The nucleus was stained with DAPI (blue). Bar 100 μm. The experiment was repeated three times and representative results from one experiment are shown.