Figure 5From: Nanobodies targeting conserved epitopes on the major outer membrane protein of Campylobacter as potential tools for control of Campylobacter colonization Interaction between Nb84 and purified MOMP. To obtain the saturation binding curve, ELISA plates were coated with purified MOMP monomers (1 µg/mL) and the interaction with increasing concentrations of His-tagged Nb84 (1 × 10−6 to 1 × 102 µg/mL) was measured. A competition assay was performed to assess the inhibition of the interaction of His-tagged Nb84 with MOMP by increasing amounts of strep-tagged Nb84. His-tagged Nb84 (5 × 10−2 µg/mL) and a serial dilution of strep-tagged Nb84 (1 × 10−6 to 1 × 102 µg/mL) were added to ELISA plates coated with MOMP (1 µg/mL). The ELISA was developed using a mouse anti-Histidine tag monoclonal antibody and goat anti-mouse IgG conjugated to alkaline phosphatase. The error bars represent the standard deviations.Back to article page